Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA.
نویسندگان
چکیده
We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.
منابع مشابه
New LightCycler PCR for rapid and sensitive quantification of parvovirus B19 DNA guides therapeutic decision-making in relapsing infections.
Detection of parvovirus B19 DNA offers diagnostic advantages over serology, particularly in persistent infections of immunocompromised patients. A rapid, novel method of B19 DNA detection and quantification is introduced. This method, a quantitative PCR assay, is based on real-time glass capillary thermocycling (LightCycler [LC]) and fluorescence resonance energy transfer (FRET). The PCR assay ...
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References 1. Zerbini M, Musiani M. Human parvoviruses. In: Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH, eds. Manual of clinical microbiology, 8th ed. Washington: ASM Press, 2003:1534–43. 2. Gallinella G, Zuffi E, Gentilomi G, Manaresi E, Venturoli S, Bonvicini F, et al. Relevance of B19 markers in serum samples for a diagnosis of parvovirus B19-correlated diseases. J Med Virol 200...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 42 7 شماره
صفحات -
تاریخ انتشار 2004